The activities of crude protocatechuate 3,4-Dioxygenase enzyme extracted from several strains of Bradyrhizobium japonicum
2001
Sakinah | Imas, T. (Institut Pertanian Bogor (Indonesia). Fakultas Matematika dan Ilmu Pengetahuan Alam)) | Achmadi, S.
The study is aimed to determine the activities of protocatechuate- 3,4-dioxygenase (PCD) extracted from Bradyrhizobium japonicum strains able to utilize aromatic compounds so that they could live competitively in soil. The work was preceeded by screening the ability of six bacterial strains to grow on basal media supplemented with three different aromatic compounds, i.e. protocatechuate acid, parahydroxybenzoic acid, and benzoic acid. Four of the six strains were able to utilize the aromatic compounds as determined using their respective enzymatic assay from crude extracts monitored by UV/VIS spectrophotometer at lamda 290 nm. Bacterial growth pattern analysis indicated that protocatechuate was the substance most easily degraded, followed by parahydroxybenzoic acid and benzoic acid respectively. The highest activity of protocatechuate 3,4-dioxygenase (PCD) crude extracts was shown by strain Bj 38 at the enzymatic activity of 0.043 U/mg, while the lowest activity was found in Bj 0.01 49 U/mg. Supplementation of 1 percent glucose into the growth media resulted in the repression of the activities of PCD crude extracts 0.009-0.041 U/mg
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