Inhibition of enteropathogenic bacteria by dichloromethane extracts derived from ginger

anglais. The investigation consisted of several experiments and analyses. Ginger was extracted with hexane, and the residue was extracted with dichloromethane, and then with ethanol. The results were three types of extracts which was non polar (hexane), semi polar (dichloromethane) and polar (ethanol). The antimicrobial activity of the extracts were determined by agar diffusion method against Escherichia coli 0157:H7, Salmonella typhi and Vibrio cholerae 01. The antimicrobial activity of the dichloromethane extract was higher than hexane and ethanol extracts. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined in ginger dichloromethane extract (GDE) that was shown as the most effective antimicrobial extract. The MIC value to E. coli 0157:H7, S. typhi and V. cholerae 01 were 10, 10 and 5 mg/ml respectively and the MBC value were 15, 20 and 8 mg/ml respectively. The ability of GDE as antimicrobial due to the nature of the extract that could easily interact with the outer membrane bacterial

indonésien. Penelitian ini dilaksanakan untuk mengetahui pengaruh polaritas ekstrak jahe terhadap aktivitas antimikroba dan menentukan nilai MIC dan MBC terhadap Esherichia coli 0157:H7, Salmonella typhi dan Vibrio cholerae 01. Hasil ekstraksi rimpang jahe secara bertahap dengan heksan, diklorometan dan etanol dihasilkan ekstrak non polar, semi polar dan polar. Aktivitas antimikroba dari ekstrak tersebut secara difusi pada nutrien agar menunjukkan bahwa semua ekstrak dapat menghambat pertumbuhan bakteri E. coli 0157:H7, S. typhi dan V. cholerae 01. Secara umum ekstrak diklorometan mempunyai penghambatan relatif yang lebih tinggi daripada ekstrak heksan dan etanol. Nilai MIC (Minimal Inhibitory Concentration) ekstrak diklorometan pada metoda pengenceran dalam NB yaitu 10, 10 dan 5 mg/ml, berturut-turut terhadap E. coli 0157:H7, S. typhi dan V. cholerae 01, dengan nilai MBC (Minimal Bactericidal Concentration) berturut-turut pada konsentrasi 15, 20 dan 8 mg/ml