Microsatellite analysis in three breeds of Indian poultry
2003
Pandey, A.K. | Mishra, B. | Chaudhary, P. | Tantia, M.S. | Vijh, R.K. (National Bureau of Animal Genetic Resources, Karnal (India))
A study was undertaken for the genetic diversity analysis using microsatellites taking 3 poultry breeds from distant regions of the country. The blood samples were collected from Nicobari, Aseel and Miri poultry randomly and DNA was isolated. The microsatellite loci were selected in such a way that they represented the complete genome and were unlinked. In all 26 microsatellite loci were used for the 3 breeds. The PCR conditions were standardized and the PCR products were run on PAGE. All the microsatellite loci were highly polymorphic with alleles ranging from 3 to 11 in the 3 populations. The polymorphic information content was more than 0.50 in all the loci indicating their suitability for genetic diversity analysis. The unbiased heterozygosity ranged from 0.5978 to 0.8825 in the entire data set and mean unbiased heterozygosity over all loci were 0.62, 0.62,0.61 for Nicobari, Miri and Aseel poultry which points towards enough scope for selection for economic traits and signifies presence of ample genetic diversity for improvement and conservation purposes.
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