Induction of suspension cultures of sunflower protoplasts
2002
Prakash, Ch.R. | Wingender, R. | Schnabl, H. (Institute of Agricultural Botany, University of Bonn, Bonn (Germany). Department of Plant Physiology and Biotechnology)
Protoplast were isolated from hypocotyls, petiols and leaves of cultivated and wild Helianthus species. Highest number of protoplasts were obtained from petiols of cultivar Euroflor i.e. 9.0 x 10(5) PP g-1 fw while lowest numbers were obtained from mesophylls of both cultivated as well as from wild helianthus sps. The isolated protoplast were cultured as liquid suspensions cultures at densities ranging from 1 x 10 (4) to 2 x 10 (5) PP ml-1 with two concentrations of BAP and NAA ie at 0.5 and 1.0 mg/l. Hypocotyl protoplasts plated at 1 x 10 (5) PP ml-1 from cultivars Apisol, Cerflor and Euroflor showed good plating efficiencies i.e., above 75% with 1.0 mg/l of BAP and NAA followed by petiol protoplasts which showed 40-47% . In case of mesophyll protoplasts divisions were observed only in cultivar Cerflor but not significantly were as mesophyll protoplasts derived from the wild species of Helianthus, ie. H. grosseserratus and h. maximillianii did not undergo any division even after 10 days and remained recalcitrant.
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