Searching by differential display for new genes implicated in induced disease resistance of cucumber [Cucumis sativus] treated with acibenzolar-S-methyl
2001
Narusaka, Y. (National Inst. of Agro-Environmental Sciences, Tsukuba, Ibaraki (Japan)) | Narusaka, M. | Ishii, H.
Acibenzolar-S-methyl (ASM) is a chemical activator of systemic disease resistance in plants. In this study, we used differential display to identify ASM-inducible defense response genes involved in induced disease resistance. As a result, we cloned three ASM-inducible genes from cucumber, encoding a chitinase, a putative protein disulfide isomerase and a putative mitochondrial-protein-like protein. Expression of these genes was induced within 24hr after treatment of cucumber leaves with ASM. These results suggest that differential display is a useful tool for understanding the mode of action of ASM and defense responses.
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