Identification of larval-stage antigens of Ascaris suum recognized with immune sera from pigs
2001
Kasuga Aoki, H. (National Inst. of Animal Health, Tsukuba, Ibaraki (Japan)) | Tsuji, N. | Suzuki, K. | Arakawa, T. | Matsumoto, Y. | Isobe, T.
Antigens were identified from the third-stage larvae (L3) and lung-stage larvae of Ascaris suttm by two-dimensional immunoblot method with antisera obtained from pigs that received chemically abbreviated Ascaris suum larval infections. Forty-seven and 13 spots were recognized as antigens from the L3 and lung-stage larvae, respectively. Their apparent molecular weight ranged from 20 to 101 kDa and their isoelectric point from 3.6 to 8.0. The present study provides a framework for further molecular cloning of those antigens and consequently leads to the development of recombinant peptide vaccines against A. suum.
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