Promotion of spore attachment of Pasteuria penetrans to Meloidogyne spp. by a combination of sonication of spores and storage of spores in water
2001
Orui, Y. (Japan Tobacco Inc., Tokyo)
Pasteuria penetrans (THORNE) SAYRE & STARR is a host-specific parasitic bacterium on the root-knot nematodes Meloidogyne spp. ( 7 ). It is known that sonication of P. penetrans spores increases the number of spores attached per second-stage juvenile (J2) of Meloidogyne spp. (4, 8) without losing their host-specific characteristic ( 4 ). Methods for identifying the major root-knot nematodes using the host-specificity of P. penetrans have recently been developed (3, 5). A large number of spores attached to J2 make the observation in these methods easy. It may become an important pretreatment for using P. penetrans to control Meloidogyne spp. The object of this study was to examine the effect of storage conditions of spores and sonication of spores on spore attachment of P. penetrans isolates to J2s of Meloidogyne species.
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