Production of virus free Japanese butterbur plants (Petasites japonicus Fr. Schmidt)
2002
"Matsumoto, J. (Hyogo-ken. Agricultural Inst., Kasai (Japan)) | Shiwaku, K. | Ohtani, R. "
"Virus free plants were obtained from flower-head tips cut from virus infected Japanese butterbur plant. (1) Shoots were induced on Murashige and Skoog's solid media containing 0.1-l.0mg/L NAA and l.0mg/L BA. The rate of shoot regeneration from flower-heads was 75-88% and even better from meristems. (2) These shoots formed roots and new shoots remarkably well on half-strength B5 solid media. Many plantlets were obtained by repeating subcultures on the same media. (3) Although filamentous virus was detected in many of the plants, 24% of the regenerated plants were virus free. (4) The growth of rhizomes increased when the virus free plants were grown in an airconditioned greenhouse in July and August."
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