Production of excellent cattle by somatic cell nuclear transfer: The second report production of the somatic cell-cloned cattle
2002
"Tozuka, Y. (Ibaraki-ken. Livestock Experiment Station, Tomobe (Japan)) | Sugawara, T. | Watanabe, A. | Nemoto, S. | Akagi, S. | Takahashi, S. | Adachi, N. | Takeda, K. | Toya, T. | Nirasawa, K. "
"The reconstructed embryos were produced by nuclear transfer cloning using the cumulus cells as the donor nuclear source that were collected by an ovum pick-up from a Japanese black cow reared at National Institute of Livestock and Grassland Science. Of 12 embryos non-surgically transferred to 12 recipient cows that were Japanese black, Holstein and hybrid at Ibaraki Prefectural Livestock Research Center, three calves were generated by a Caesarian birth. DNA analyses confirmed that these calves were genetically identical to the same donor cow. Birth weights of three calves were 31.0 kg, 37.5 kg and 31.0 kg, respectively, and almost the same as the standard birth weight of Japanese black females. All calves had the thick navel cord, and the navel tissue of one calf was weak and breakable."
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