Ribonuclease production of Rhizopus oryzae IFO 4697 under metal ion stress in liquid culture
2002
"Morita, H. (University of Kitakyushu, Fukuoka (Japan)) | Shiozawa, M. | Fujio, Y. "
"Rhizopus oryzae IFO 4697 was found to produce intracellular ribonuclease (RNase), and its growth and activity could be regulated under selected metal ion stress. The addition of Fe**2+, Mg**2+ and Zn**2+ to the SLSR medium was essential to growth and RNase production. Ca**2+ and Mo**6+ stimulated RNase production. It is concluded that the addition of 100mg/ml Ca**2+, 5 mg/ml Mo**6+, 0.7 mg/ml Zn**2+, 2mg/ml Fe**2+, and 49mg/ml Mg**2+ to the SLSR medium was the best condition for producing RNase in high specific activity (3780U/mg protein). This result indicates that a metal ion-regulated liquid medium is an efficient culture method for RNase production. "
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