A technique to enrich alpha-tocopherol in Chlorella and marine rotifers
2003
Nagano, N. (Miyazaki Univ. (Japan). Faculty of Agriculture) | Hayashi, M. | Yukino, T. | Murata, H.
a-Tocopherol (TOC) and various fatty acids were supplemented into the Chlorella vulgaris strain K-22 culture at 28 deg C for 24 h, and TOC content of the Chlorella cells was determined. The addition of TOC alone into the Chlorella culture medium did not give an increase of the cellular TOC content However, the TOC contents in the Chlorella cells supplemented with TOC and various fatty acids were much higher than those supplemented with TOC alone. Moreover, the TOC content in the cells supplemented with a mixture of TOC and oleic acid was higher than those supplemented with a mixture of TOC and docosahexaenoic acid (DHA) . The TOC content of the rotifers fed on the TOC-enriched Chlorella was much higher than rotifers fed on unenriched Chlorella. On the other hand, rotifers could be easily enriched with TOC by feeding the TOC-enriched Chlorella cells.
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