Effect of alpha-cyclodextrin-allyl isothiocyanate on ruminal microbial methane production in vitro
2003
Lila, Z.A. (Tokyo Univ. of Agriculture and Technology, Fuchu (Japan). Faculty of Agriculture) | Mohammed, N. | Kanda, S. | Kamada, T. | Itabashi, H.
The objective of the present study was to investigate the effects of ??-cyclodextrin-allyl isothiocyanate (CD-Al) on ruminal microbial methane production and rumen fermentation of corn starch, soluble potato starch or hay plus concentrate (1 .5:1) by mixed rumen microorganisms. Diluted rumen fluid (30 mL) was incubated anaerobically at 38 deg C for 6 and 24 h with or without CD-AI (O, 0.4, 0.8, 1.6 and 3.2 g/L). The pH of the medium was unchanged by CD-AI in all substrates. The molar proportion of acetate was decreased and propionate was increased with a corresponding decrease in acetate : propionate ratio (P< 0.05). Total volatile fatty acids and butyrate were increased (P < 0.05). Ammonia-N was decreased (P < 0.05). Except with soluble potato starch, numbers of protozoa were unchanged after 6 h. As concentration of CD-Al increased from 0 to 3.2 g/L, fermentation of corn starch, soluble potato starch and hay plus concentrate resulted in decreased (P < 0.05) methane production of 49-100% (6 h) and l4-100% (24 h): 39-100% (6 h) and 16-100% (24 h); and 45-100% (6 h) and 17-100% (24 h), respectively. When hay plus concentrate was used as substrate, methanogenic bacteria were decreased (P < 0.05) with 0.8 g/L of CD-Al after 6 h. Excluding the lower dose level (0.4 g/L) of CD-Al, digestibility of neutral detergent fiber of hay plus concentrate was decreased (P < 0.05) after 24 h. A suitable level of CD-Al could therefore be used as a supplement to inhibit methane production and improve rumen fermentation without detrimental effects on fiber digestion.
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