Identification method of mutations in non-coding regions of chloroplast genome of cogongrass (Imperata cylindrica) by PCR-RFLP technique
2003
Yasuda, K. (Saga Univ. (Japan)) | Shibayama, H.
Summary Cogongrass (Imperata cylindrica) is a perennial common in human disturbed environment such as paddy levees, roadsides, and slopes of embankment. It is one of important plant species for the study of conservation genetics at human disturbed environment. Previous study using sequencing technique found nucleotide mutations at 7 sites (5 substitutions and 2 indels) in psbA-matK intergenetic spacer, trnV intron, and psaC-ndhE intergenetic spacer regions of cogongrass chloroplast genome. PCR-RFLP is " a convenient method for detecting nucleotide mutation. If we can identified mutation pattern at the 7 sites by PCR-RFLP technique instead of the sequencing, genetic variation of a large number of samples will be evaluated efficiently. We reported identification method of mutation patterns at the 7 sites in three non-coding regions of cogongrass chloroplast genome by PCR-RFLP technique.
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