Genetic Differences and DNA Polymorphism in Oyster (Crassostrea spp.) Analysed by RAPD-PCR
2004
Kim, J.Y. (Kunsan National University, Kunsan, Republic of Korea) | Park, C.Y. (Kunsan National University, Kunsan, Republic of Korea) | Yoon, J.M. (Kunsan National University, Kunsan, Republic of Korea), E-mail: [email protected]
Seven oligonucleotides decamer primers of the 20 arbitrarily chosen primers were used on the basis of the number of the polymorphisms generated in oyster (Crassostrea gigas) from Buan and Geojedo. Engaged to amplify genomic DNA isolated from the muscle of individual oysters, the random primers OPA-08, -09, -16, -17, -20, OPB-03 and -07 generated common polymorphic and specific bands. The selected 7 primers generated the total of 481 distinguishable fragments in the oyster population from Buan and 264 in the population from Geojedo.
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