Studies on spread and identification of zucchini yellow mosaicvirus disease in the north-west mediterranean region of Turkey bybiological indexing and double-stranded RNA analysis
2004
Yardimei, N.,University of Suleyman Demirel, Isparta (Turkey).Dept. of Plant Protection
Leaf and fruit samples showing symptoms of zucchini yellowmosaic virus (ZYMV) disease were collected from cucurbit fields inIsparta province in North-West Mediterranean region of Turkey.Mechanical inoculations on test plant were made by conventional leaf-inoculation method. The virus was multiplied in squash plant(Cucurbita pepo (L.)) and showed systemic symptom. Double-strandedRNA (dsRNA) was purified by CF-11 cellulose chromatography. Agarosegel electrophoresis indicated that dsRNA was present in infectedsquash plants. The disease rate of ZYMV was determined by countingthe plants showing the disease symptoms in 20 cucurbit fields inIsparta. Based on our surveys the infection ZYMV was determined atthe rate of 62.70% in the squash field.
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