Relationship between vascular endothelial growth factor and Interleukin-6 in diabeticretinopathy
2004
Baiomy, A.A. | Arafa, L.M.,Mansoura Univ. (Egypt). Dept. of Medical Biochemistry)Ghanem,A.A
The present study has to investigated the relationship between diabetic retinopathy andthe levels of vascular endothelial growth factor and IL-6 in aqueous humor and plasma andalso determination of VEGF gene expression. The current study was carried out on undilutedaqueous humor samples and the corresponding plasma samples of 24 diabetic patients (24 eyes)and 12 non diabetic patients (12 eyes) who underwent cataract surgery. VEGF and IL-6 weremeasured in aqueous humor and plasma samples by Enzyme Linked Immunosorbent Assay(ELISA) kits and total RNA content was measured and VEGF gene expression was analyzed byRT-PCR. VEGF and IL-6 levels revealed a high significant rise in aqueous humor of diabeticpatients and non-significant rise in plasma of the same patients. A high +ve correlation wasfound between VEGF and IL-6 in aqueous humor samples of diabetic patients. In addition,present results revealed increased expression of three molecular forms of VEGF (VEGF121,VEGF145, VEGF165) in diabetic patients. VEGF and IL-6 are produced together in theintraocular tissues and are involved in the pathogenesis of DR. VEGF gene expression isincreased early in DR. So, anti-VEGF drugs may have future application for treatment ofdiabetic retinopathy.
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