Assessment of genetic variability in some Iranian sweet oranges(Citrus sinensis (L.) Osbeck) and mandarins (Citrus reticulata Blanco)using SSR markers
2005
Golein, B.,Tehran University, Karaj (Iran). Dept. of HorticulturalScience
The genetic variability of eight sweet oranges (Citrus sinensis [L.]Osbeck) and six mandarins (Citrus reticulata Blanco) accessions wasevaluated using simple sequence repeats (SSRs) analysis. In total, 52putative alleles were detected using seven primer pairs. The number ofputative alleles per primer pair ranged from 3 to 10 with an average of7.42. Polymorphic information content (PIC) value changed from 0.505 to0.950. Microsatellite markers discriminated variation within mandarins, butlow variation observed between sweet oranges. A UPGMA phenetic treewas constructed and one main sweet orange group consisting of threesub- groups and four main mandarin groups were identified. Themajority of sweet orange accessions showed a narrow genetic basesuggesting that the observed morphological polymorphism within thegroup must be associated with somatic mutations, which were not exactlydetected by these molecular markers.
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