Improved methods for anther and pollen culture in rice
2003
Gill, R. | Kaur, N. | Sindhu, A.S. | Bharaj, T.S. | Gosal, S.S.
Improved methods have been developed for anther and pollen culture from diverse genotypes, including basmati and tropical japonica types, high-yielding indica x new plant type, and Bt basmati. In general, the use of N6 medium supplemented with 2,4-D(2.5 mg L¯¹), proline (560 mg L¯¹), and maltose (30 g L¯¹) has resulted in better callusing. The use of antibiotics cefotaxime (500 mg L¯¹) and cysteine (25 mg L¯¹) induced pollen embryogenesis in some cases. Embedding of anthers in agarose was better than culturing of anthers on semisolid medium or in liquid medium. Likewise, culturing of pollen on the filter membrane, overlying rice feeder cells, or embedding of isolate pollen in agarose has resulted in a significant increase in the number of pollen calli in Hsinchu 64 and IR-65598-112-2. These methods are now being employed for producing homozygous Bt basmati through anther/pollen culture from transgenic basmati (T1 plants) carrying cry1A(c).
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