Effect of maltose on anther culture of Tongil and indica rice
2003
Yi, G.H. | Nam, M.H. | Oh, B.G. | Kim, H.Y.
The low frequency of plant regeneration from anther culture of some genotypes such as Tongil and indica rice is one of the major problems in the practical use of this technique in rice breeding. This study examined the effect of maltose on callus formation and plant regeneration in anther culture of these recalcitrant varieties. Maltose increases anther response not only in Tongil and indica rice but also in japonica varieties. Interestingly, the percent callus induction was higher in Tongil and indica than in japonica varieties. Callus induction in indica was 2- to 14-fold higher in maltose (40-60 g L¯¹) supplemented medium than in sucrose plus glucose supplementation medium.
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