Use of ELISA technique for the detection of antibodies against Salmonella enteritidis in chicken flocks
2000
Indhira Kramomtong | Waree Niyomthum | Kriengsuk Poonsuk | Wanpen Chaicumpa
Two experiments were carried out in 8 days to 13 weeks and 12 weeks to 20 weeks old chicken. All chicks were experimentally infected by oral inoculation with one milliliter of 105 CFU S. enteritidis. Sera were collected once a week for detection of antibodies to S. enteritidis as well as cloacal swabbing for bacterial isolation. Experiment 1. In young chicken, S. enteritidis was isolated from faecal excretion every weeks. By using IHA, one chicken (1/16) was positive at week 4 and the other (1/16) at week 11 following inoculation. Whereas two chicken (2/16) were identified positive after week 4, and 12 to 36 percent of the samples were found seropositive between the period of week 7 to 12 by ELISA technique. Experiment 2. In adult chicken, S. enteritidis was isolated from faecal excretion of one chicken (1/58) at the week and other (1/42) at week 6. Seropositive by IHA test was detected after the first week and continuously detected in 8.5 to 37.5 percent of the chickens through 8 weeks of the experiment. Similarly, ELISA was detected from the first week to 12 weeks and ranged 29.8 to 65.6 percent. The results showed that ELISA was more sensitive than IHA and possibly used to indicate the disease condition in the flock of over 8 weeks old chicken. There was a linear relation between ELISA and IHA but not bacterial isolation. In conclusion, our result suggests that ELISA is suitable for serological survey to S. enteritidis.
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