Growth and enzyme activities of Neocallimastix frontalis McH3 using pineapple peel as substrate
1999
Pornpan Lerstaveesin
Neocallimastix frontalis McH3 grew anaerobically in various carbon sources such as cellulose, CMC, xylan and pineapple peel. The fungus produced 14.99, 33.26, 33.78, 19.68 mM of hydrogen, acetate, formate and lactate respectively when grown on 4 g/l of pineapple peel containing media. The fermentation products of N. frontalis McH3 from solid biomass contains large amounts of acidogen which are very useful substrate for methane fermentation. The cellulase and xylanase activities detected in the culture filtrate, were 1.77 and 1.83 IU/ml respectively. The pineapple peel consumption was 56.54 percent at 8 days of incubation. Thus the experiments concerning bioconversion of pineapple peel to methane with co-culture of N. frontalis McH3 and methanogenic bacteria should be further studied.
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