SDS-page analysis for protein patterns of adults Haemonchus placei, Mecistocirrus digitatus and Cooperia spp. in cattle
2000
Sonthaya Tiawsirisup(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Sciences. Department of Veterinary Pathology. Veterinary Parasitology Unit) | Amonthep Khuprathumsiri(Chulalongkorn Univ, Bangkok (Thailand). Faculty of Veterinary Sciences. Large Animal Hospital) | Suwannee Nithiuthai(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Sciences. Department of Veterinary Pathology. Veterinary Parasitology Unit) | Nikom Chaisiri(Chulalongkorn Univ., Bangkok (Thailand). Faculty of Veterinary Sciences. Dept. of Veterinary Physiology. Biochemistry Unit) | Thirayuth Kositamongkol(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Sciences. Department of Veterinary Pathology. Veterinary Parasitology Unit) | Sudchit Chungpivat(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Sciences. Department of Veterinary Pathology. Veterinary Parasitology Unit)
Protein profiles of crude extracts from adult male and female Haemonchus placei, Mecistocirrus digitatus and Cooperia spp. Were studied. The parasites were collected from the abomasums and duodenum of cattle at Pathumthani and Nonthaburi abattoirs. SDS-PAGE analysis of the crude extracts revealed, protein bands of various intensities ranging from 13-24 bands with a molecular mass of between 19-130 kDa. The protein band patterns of each parasite at a molecular weight of between 48.2-111 kDa were similar whereas other protein bands at a molecular mass below 48.2 kDa were different. The number of distinct bands shown by the crude extract from males and females of three species were 13 and 21, 20 and 18 and 24 and 23 respectively. High density, specific protein bands from Mecistocirrus digitatus and Cooperia spp. Were clearly shown at 22 and 20/ 20.5 kDa. In contrast, a low density but specific band at 33.8 kDa was only seen in extract from the male adult Haemonchus placei.
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