Vegetable seed and excised embryo cryopreservation
1999
Panie Tongpamnak | Montha Wongmaneeroj | Prathuang Donsomphrai | Pangluang Choipoi (Kasetsart Univ., Bangkok (Thailand). Research and Development Institute)
Cryopreservation techniques were conducted using seeds of several kinds of vegetable growing in Thailand stored in liquid nitrogen (temperature -196 deg C.) The results revealed that the seed moisture content should be lower than normal. Then the seeds were kept in a cryotube that can withstand ultra low temperature of liquid nitrogen. The seeds of sweet basil, chilli, tomato, sweet corn, okra, occumber, edible rapes, chinese kale and yard long bean could be taken out from the liquid nitrogen tank and sown in the field with 5-7 percent germination rate lower than the control. The excised embryo can also be conserved in a liquid nitrogen. The yard long bean seeds are allowed to soak in water for 24 hours at 25 deg C. The embryos were excised, desiccated in an aseptic jar. The embryos were pre-cooled at -40 deg C for 2 minutes before keeping in liquid nitrogen. The embryos can be conserved as long as there is liquid nitrogen in the tank. To germinate, the embryos were allowed to thaw at 40 deg C for 16 hours and cultured in an MS medium. The seedlings were transferred to the greenhouse for hardening, before transplanting in open field.
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