Genetic divergence and variability of rapeseed (Brassica napus L.) seed yield components
2005
Marjanovic-Jeromelo, A.
The morphological and biochemical traits (plant height, height to the first branch, stem diameter, branch number, leaf number, pood number, seed yield per plant, 1000-seed weight, oil content, and protein content), as well as, molecular (RAPD) markers were analysed in order to determine genetic distance between 30 winter rapeseed genotypes. 9 out of 13 RAPD primers that were used for the analysis enabled estimation of DNA polymorphism of 30 winter rapeseed genotypes, while the other primers were monomorphic. Dendrograms were obtained with the use of cluster analysis and different methodological procedures for determination of genetic distance were compared. Dendrograms for certain quantitative traits, as well as, dendrogram obtained with RAPD markers show partial grouping of genotypes based on their geographical origin. Interaction genotype/environment of the tested genotypes was determined with the use of AMMI analysis. The greatest influence on the expression of tested traits in rapeseed had the environment, i.e. year, except for the seed protein content, where the genotype had the greatest influence. Interaction genotype/environment was also highly significant for all traits. Graphical presentation of AMMI in the form of biplot was used. This could facilitate the choice of stable genotypes with the high values for the desired trait or trait combination.
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