Determination of biotin content in beet molasses by Lactobacillus plantarum
2005
Loncar, E.S. | Dosenovic, I.S. | Markov, S.L. | Malbasa, R.V. | Kolarov, Lj.A.(University of Novi Sad, Novi Sad (Serbia and Montenegro). Faculty of Technology)
D-biotin content in beet molasses was determined by microbiological method using Lactobacillus plantarum based on the comparison of the growth of this microorganism in molasses solutions with those in standard solutions of biotin. Incubation of the microorganism was performed on original Vitamin Biotin Testbouillon and laboratory prepared liquid culture medias. The amount of real biotin in molasses is low. The results depend upon the sample and volume of molasses solutions. Biotin contents obtained on both liquid media are close.
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