Cytokine production by porcine mononuclear leukocytes stimulated by mitogens
Raskova, G.(Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Fyziologie)E-mail:[email protected] | Kovaru, F.(Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Fyziologie) | Bartova, J.(Vyzkumny Ustav Stomatologicky, Prague (Czech Republic))
The aim of the study was to contribute to the understanding of the postnatal development of the immune system in domestic pigs by studying in vitro cytokine production by mononuclear leukocytes stimulated with pokeweed mitogen (PWM) and concanavalin A (ConA). The production of interleukin 1beta (IL-1beta), interferon gamma (IFN-gamma), and tumor necrosis factor alpha (TNF-alpha) was evaluated. The mitogenic effect was studied in cultures of two different cell concentrations (100,000/mL and 200,000/mL) and assessed by the ELISpot method. The stimulatory effect was detected in IL-1beta-secreting cells after exposure to PWM and ConA. IFN-gamma-secreting cells at the concentration of 100,000 cells/mL responded to PWM to a low degree. The porcine immune system at 60 days postnatally is mature enough to be able to selectively control the response of cytokine producing cells to mitogenic stimulation.
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