Role of phytohormones in organogenic ability of elm multiplicated shoots
2006
Mala, J.(Vyzkumny Ustav Lesniho Hospodarstvi a Myslivosti, Prague (Czech Republic)) | Gaudinova, A.(Akademie Ved, Prague (Czech Republic). Ustav Experimentalni Botaniky) | Dobrev, P.(Akademie Ved, Prague (Czech Republic). Ustav Experimentalni Botaniky) | Eder, J.(Akademie Ved, Prague (Czech Republic). Ustav Experimentalni Botaniky) | Cvikrova, M.(Akademie Ved, Prague (Czech Republic). Ustav Experimentalni Botaniky) E-mail:[email protected]
A comparative analyses of endogenous contents of auxin (IAA), cytokinins (CKs), polyamines (PAs), and phenolic acids (PhAs) in apical and basal parts of elm multiplicated shoots with regard to the organogenic potential is presented. The shoot-forming capacity was higher in the apical part than in the basal part. Root formation was in the apical type of explant significantly delayed. Significantly higher concentrations of free bases, ribosides and ribotides of isopentenyl adenine, zeatin and dihydrozeatin were found in apical segments in comparison with basal ones. In comparison with basal parts, apical shoot parts were characterised by higher putrescine and spermidine contents and by approximately three times higher concentrations of endogenous free IAA. The predominant PhA in both types of explants was caffeic acid. Higher concentrations of glycoside-bound p-coumaric, ferulic and sinapic acids were found in basal than in apical parts.
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