IL-10 augments antibody production in in vitro immunized lymphocytes by inducing a Th2-type response and B cell maturation
2004
Xu, Q. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) | Katakura, Y. | Yamashita, M. | Fang, S. | Tamura, T. | Matsumoto, S. | Aiba, Y. | Teruya, K. | Osada, K. | Nishikawa, R. | Shirahata, S.
An in vitro immunization (IVI) protocol enables antigen specific antibody production from L-Leucyl-L Leudse methyl ester (LLME)-treated human peripheral blood lymphocytes (PBL) upon antigen stimulation In the presence of IL-2, IL-4, and muramyl dipeptide. In the course of our studies, we have found that IL-10 added at the antigen sensitization significantly augmented antibody production level from the LLME-treated PBL. In the present study, we tried to demonstrate the role of IL-10 In the augmentation of antibody production in an IV[ protocol by clarifying the cytokine expression profiles In CD4+ and CD8+ T cells. The results showed that IL-10 skewed the Th1/Th2 balance to Th2-type responses by suppressing Thl-type cytokine production and augmenting Th2-type cytokine production In CD4+ and CD8+ T cells, as well as in CD19+ B cells Furthermore, IL-10 augmented the expression of CD3g, an antigen marker of plasma cells, on B cells, which clearly Indicates that IL-10 promoted differentiation and maturation of B cells In an WI protocol. These results Indicate that IL-10 plays an important role in setting the cellular milieu to produce antibodies In an IVI protocol.
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