Method for estimation of toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice
2004
Takikawa, N. (Kitasato Inst., Tokyo (Japan)) | Kawahara, K. | Sawata, A. | Kume, K.
We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the injection. The best result was obtained when C3H/HeN mice were used for the test. Correlation was observed between the endotoxin content measured by Limulus amoebocyte lysate assay and the LD sub(50) in the mouse safety test (r=O.81). These results suggested that this test could be applied to the estimation of endotoxin content in inactivated vaccines of Salmonella.
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