Life cycle of Arabidopsis (Arabidopsis thaliana) plant in vitro
2005
Iwasaki, N. (Tsukuba Univ., Ibaraki (Japan)) | Sato, Y. | Hisajima, S.
The establishment of the plant life cycle in vitro, alteration of generations in vitro and/or cross breeding in vitro will stimulate the development of a breeding-oriented plant factory. Reports on plant life cycles in vitro are limited. In the present research, the complete life cycle of Arabidopsis thaliana was examined by culturing seeds under different conditions that included solidifying agent, temperature, vessel and air permeable membrane. When Arabidopsis seeds were cultured in 225 ml culture bottles with an air permeable membrane containing the Murashige and Skoog's medium, 30g/l sucrose and 8g/l agar at 22 deg C under a 16 h photoperiod with 35 micro moles of photosynthetic available photons/square m/sec, 100% of the cultures formed flowers and 67 % of the cultures produced seeds indicating that the life cycle of Arabidopsis was completed in vitro. One hundred % of the in vitro produced seeds were germinated in potted soil indicating that the in vitro produced seeds were viable. Young plants from the seeds were grown in potted soil and were morphologically normal. The in vitro produced seeds germinated and plants produced flowers in vitro, and the in vitro 3rd generation seeds were produced under the same conditions as the 2nd generation seeds were born in vitro. The present system may stimulate research on the plant life cycle in vitro, and be used as a general leading model.
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