Effect of the well of the well (WOW) system on in vitro culture for porcine embryos after intracytoplasmic sperm injection
2005
Taka, M.(Obihiro Univ. of Agriculture and Veterinary Medicine, Hokkaido (Japan)) | Iwayama, H. | Fukui, Y.
For developmental competence of porcine embryos in vitro, it is important to improve the culture environment. The present study was performed to evaluate four different culture systems for in vitro matured porcine oocytes following intracytoplasmic sperm injection (ICSI); drop, well and two sizes of the well of the well (WOW) systems (500 and 1,000 mu m in diameter). The cleavage rate on Day 2 and the mean cell number in blastocysts on Day 6 were not significantly different among the four treatments. However, the 1,000 mu m WOW (24.6%) resulted in a significantly higher (P0.05) blastocyst rate than those in the other culture systems (12.9, 14.8, and 7.1% for drop, well, and 500 mu m WOW, respectively). The present study indicates that the microenvironment created by the 1,000 mu m diameter WOW improves blastocyst produdion of in vitro matured porcine oocytes after ICSI, and that the effectiveness of the WOW system is dependent on the size (diameter) of the WOW.
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