Characterization of expressed genes in kidney cells of Japanese flounder Paralichthys olivaceus following treatment with ConA/PMS and LPS
2004
Arma, N.R.(Tokyo Univ. of Marine Science and Technology (Japan)) | Hirono, I. | Aoki, T.
Two cDNA libraries were prepared from Japanese flounder Paralichthys olivaceus kidney cells that had been treated with LPS or a combination of ConA and PMA. From these two libraries, we sequenced 373 and 379 clones, respectively. Of these 752 clones, 109 clones were characterized as immune-related. Among the immune-related CDNAS, the following have not been previously reported in fish: NK-Iysin, perforin, complement C1q, CD9 antigen, CD63 antigen, CD82 antigen, ISGF-3, IP-30, G-CSF, equistatin precursor, TLS-CHOP, fas ligand and CARD4. This study highlights the success of using ConA/PAM and LPS as mitogens to increase the number of immune-related genes expressed in Japanese flounder and demonstrates that different stimuli induce the expression of different immune-related genes.
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