Polyamine analysis within the eubacterial thirteen phyla Acidobacteria, Actinobacteria, Chlorobi, Chloroflexi, Chrysiogenetes, Deferribacteres, Fibrobacteres, Firmicutes, Fusobacteria, Gemmatimonadetes, Nitrospirae, Planctomycetes, and Verrucomicrobia

Cellular polyamines of the recently described 51 eubacteria distributed within 13 phyla were analyzed by HPLC, to demonstrate polyamine profiles in numerous eubacterial taxa of different lines of descent. Homospermidine or norspermidine was found within the phyla Acidobacteria. Chloroflexi and Chlorobi. In the phylum Nitrospirae, mesophilic Nitrospira and Leptospirillum contained spermidine alone, whereas thermophilic Thermodesulfovibrio contained norspermine, spermine and homocaldopentamine. The phylum Chrysiogenetes contained spermidine alone, and the phylum Deferribacteres cadaverine, spermidine and spermine, and the phylum Fibrobacteres spermidine. Spermidine and spermine were distributed within the phylum Fusobacteria. The phylum Gemmatimonadetes contained homospermidine alone. Within the phylum Planctomycetes, homospermidine was the major polyamine. The phylum Verrucomicrobia contained spermidine alone. In the phylum Firmicutes, the mesophiles of the order Clostridiales contained low levels of cadaverine, spermidine, spermine and agmatine and thermophilic Heliobacterium was rich in spermine. The order Halanaerobiales contained spermidine and spermine. In the orders Bacillales and Lactobacillales, many mesophiles contained spermidine and agmatine and some lacked polyamines. In the phylum Actinobacteria, the orders Rubrobacterales, Coribacteriales and Sphaerobacterales contained no cellular polyamines whereas thermophilic Thermoleophilum contained branched penta-amines. The eubacteria tested can be characterized by major triamine profiles (spermidine, norspermidine and/or homospermidine), roughly corresponding to their phylogenetic positions at class or order level. Tetra-amines and penta-amines appeared in thermophiles. Psychrophily, halophily, acidophily and alkaliphily found in some eubacteria within the 13 phyla seem to be not related to their polyamine profiles.