Primary structure and mRNA expression of carp (Cyprinus carpio) cathepsin B
2006
Tan, Y.(Nagasaki Univ. (Japan). Faculty of Fisheries) | Osatomi, K. | Nagae, M. | Hara, K.
We cloned a cathepsin B cDNA from the muscle of carp. The nucleotide sequence of carp cathepsin B cDNA consisted of 1470 bp including a 993 bp open reading frame, encoding a deduced protein of 330 amino acids. The deduced amino acid sequence of carp cathepsin B has similarity of 80 % to that of rainbow trout cathepsin B and of 76-79% to mammalian cathepsins B. Based on a multiple sequence alignment, carp cathepsin B was considered to work in two-chain form consisting of a light chain and a heavy chain. Tissue distribution of the carp cathepsin B mRNA has been studied by northern blot analysis. The result revealed that cathepsin B was constitutively expressed in every tissue, and the relative amount of cathepsin B mRNA expression showed decreased in the order of ovary, gill, intestine, testis, hepatopancreas, spleen, kidney, heart, and brain. A high expression of cathepsin B transcripts in ovary might be necessary for yolk formation.
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