Liposome-Mediated Electric Gene Delivery into Fetal and Adult Gonads
Choi, S.C.;Choi, S.K.;Choi, S.S.;Kim, S.U.;Cho, N.N.;Jung, J.Y.;Lee, S.H.(Korea University, Seoul, Republic of Korea)E-mail:sangho@korea.ac.kr | Park, C.S.(Chungnam National University, Daejeon, Republic of Korea) | Lee, S.H.(Kongju University, Kongju, Republic of Korea)
Gene delivery is one of the keen interests in animal industry as well as research on gene functions. Some of the in vivo gene delivery techniques have been successively used in various tissues for the gene therapy and transgenesis. Despite intensive efforts, it still remains to overcome problems of limited local and regional administration and low transgene expression. To improve the efficiency of gene delivery, a new procedure was tested. We injected exogenous DNA containing LacZ into the female or male gonads and then pulsed electric field. Electroporated gonads showed positive X-gal staining in many seminiferous tubules of the porcine fetal gonads. Exogenously introduced LacZ genes were also expressed in female porcine gonad.
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