Separation and Performance lest of Wnitening Agent in Rhodiola Sachalinensis
Choi, D.Y.;Ahn, S.Y.;Lee, S.G.;Han, J.S.;Kim, E.C.;Lee, H.B.;Kim, E.K.;Row, K.H.(Inha University, Incheon, Republic of Korea)E-mail:[email protected] | Shin, J.H.(Inha University Hospital, Incheon, Republic of Korea)
The Rhodiola sachalinensis 5 g were mixed and extracted with methanol 150 ㎖ at the room temperature for 12 h. The effluents were collected and grouped into the two. In this experimental condition, the mobile phase composition were linearly changed as followswater/methanol90/10 - 30/70 (vol. %, for 5 min), 30/70 - 10/90 (vol. %, for 15 min) and an analytical column (3.9 × 25 cm, 15 ㎛ particle size, and 300 Å pore size) was utilized. The performance of the extracted Rhodiola Sachalinensis as a whitening agent was not favorable, so it classifies the Rhodiola Sachalinensis extractions with two fractions and collects each fraction for whitening agent assay.
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