T-DNA Integration Patterns in Transgenic Tobacco Plants
Lee, M.K.;Kim, H.S.;Kim, S.H.;Park, Y.D.(Kyung Hee University, Yongin, Republic of Korea)E-mail:ydpark@khu.ac.kr
To investigate the various integration patterns of T-DNA generated by infection with Agrobacterium, we developed a vector (pRCV2) for the effective T-DNA tagging and applied it to tobacco (Nicotiana tabacum cv. Havana SR1). pRCV2 was constructed for isolating not only intact T-DNA inserts containing both side borders of T-DNA, but also for partial T-DNA inserts that comprise only the right or left side. We also designed PCR confirmation primer sets that can amplify in several important regions within pRCV2 to detect various unpredictable integration patterns.
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