Development of PCR-dot blot hybridization for the diagnosis of alcelaphine herpesvirus 1
Kim, O.J.(Seoul National University, Seoul, Republic of Korea)E-mail:kimoj@snu.ac.kr | Li, H.(USDA-ARS, Animal Disease Research Unit, Pullman, WA, USA)
The aim of the present study was to develop a sensitive and specific assay for the diagnosis of alcelaphine herpesvirus 1 (AlHV-1) which is a cause agent of malignant catarrhal fever in ruminants. A1HV-1 is a gamma herpesvirus, which is frequent latent, and it is often difficult to detect its antigens or specific nucleic acids because of its low genomic copies in the infected tissues. In this study, polymerase chain reaction (PCR)-dot blot hybridization (DBH) assay for detecting AlHV-1 DNA was developed and evaluated for its sensitivity and specificity as comparison with PCR and DBH alone. The developed PCR-DBH was more sensitive than PCR or DBH alone and also very specific.
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