Production of expressed protein from cloned Shigatoxin 2e gene and Receptor Binding Affinity of the toxin
Dong, B.Y.(Korea Green Cross Co., Yongin, Republic of Korea) | Kim, S.H.(University of Guelph, Guelph, Ontario, Canada) | Kim, Y.I.(National Veterinary Research and Quarantine Service, Busan, Republic of Korea) | Cho, H.H.(National Veterinary Research and Quarantine Service, Seoul, Republic of Korea) | Lee, W.W.(Pusan Institute of Health and Environment, Busan, Republic of Korea) | Kim, K.S.;Kang, H.J.;Kim, Y.H.(Kyeongsang National University, Jinju, Republic of Korea)E-mail:yho157@nongae.gsnu.ac.kr
This study was designed to determine optimal condition for expression of cloned Shigatoxin2e(Stx2e) gene from transformed E. coli PED18, to compare the cytotoxicity titer between cloned Stx2e and Stx2e from original strain, and to confirm of receptor binding affinity of Stx2e for use of development of receptor binding ELISA to detect of Stx2e. The optimum composition of medium for expression of Stx2e gene in E.coli host-vector system was definded as the medium containing 0.5% glucose and 0.5 mM IPTG. The cytotoxicity titer of expressed Stx2e for Vero cell was 1000 fold higher than that of Stx2e from original strain AY93258.
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