Establishment of micronuclus assay as biological dosimetry in pig lymphocytes after gamma-irradiation
Kim, S.R.;Lee, H.J.;Lee, J.H.;Kim, J.C.;Kim, S.H.(Chonnam National University, Gwangju, Republic of Korea)E-mail:shokim@chonnam.ac.kr | Kang, C.M.(Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea) | Kim, T.H.(Kyungpook National University, Daegu, Republic of Korea) | Jo, S.K.(Korea Atomic Energy Research Institute, Daejeon, Republic of Korea)
The purpose of the present experiment was to investigate the micronuclei(MN) frequency in cytokinesis-blocked(CB) cells after various doses of gamma-rays in pig (Landrace, male, 3-month-old) and so to contribute to the clarification of the question whether these species are suitable as a target organism in the test system. The frequencies of binucleated cells, and gamma-ray-induced MN in CB cells at several doses were measured in three donors. The peaks of binucleated lymphocyte formation(22%) were found at a concentration of 2% phytohaemagglutinin(PHA) and 4 ㎍/ml Cytochalasin B(Cyt-B) in pig at 72 hours after incubation.
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