PVY Resistant Transgenic Potato Plants (cv Claustar) Expressing the Viral Coat Protein
2005
Gargouri-Bouzid, Radhia (Ecole Nationale d' Ingenieurs de Sfax, Sfax, Tunisia), E-mail: [email protected] | Jaoua, Leila (Institut National de la recherche Scientifique de Tunis(INRST), Sfax, Tunisia) | Mansour, Riadh Ben (Centre de Biotechnologie de Sfax, Sfax, Tunisia) | Hathat, Yemna (Centre de Biotechnologie de Sfax, Sfax, Tunisia) | Ayadi, Malika (Centre de Biotechnologie de Sfax, Sfax, Tunisia) | Ellouz, Radhouane (Centre de Biotechnologie de Sfax, Sfax, Tunisia)
The coat protein mediated resistance to potato virus Y is assessed here in transgenic potato plants (Solanum tuberosum L., cv Claustar). Therefore, the corresponding cDNA from tunisian isolate of the virus was cloned into Agrobacterium tumefaciens binary vector. The transgenic lines were subsequently analysed for the presence and expression of the transgene. The CP cDNA copy number was determined for kanamycin resistant plants. Three selected transgenic lines and their S1 progeny resulting from tuber germination showed a high protection level against the virus.
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