Direct Somatic Embryogenesis of Curculigo orchioides Gaertn., an Endangered Medicinal Herb
2004
Thomas, T. Dennis (St. Thomas College, Pala, Kerala, India), E-mail: [email protected] | Jacob, Alphonsa (St. Thomas College, Pala, Kerala, India)
In vitro multiplication of Curculigo orchioides was achieved by direct somatic embryogenesis in young leaf segments. Immature leaf segments of about 0.5 cm in length were cultured on MS medium supplemented with different concentrations of BAP (2-10 μM) or Kin (2-10 μM). Optimum response in terms of per cent cultures responding (89%) and the number of embryos per explant (16) were observed on MS medium supplemented with 8 μM BAP. The emergence of several somatic embryos on the adaxial side of the leaf segments was observed one month after the culture.
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