Identification of S-haplotype Based on Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms and Sequence Analysis of the S-Locus Receptor Kinase Gene in Chinese Cabbage Inbred Lines
2004
Seo, J.P. (National Agricultural Cooperative Federation, Ansung, Republic of Korea) | Kim, H.J. (Nara Institute of Science and Technology, Ikoma, Japan) | Nou, I.S. (Sunchon National University, Sunchon, Republic of Korea)
Identification and DNA polymorphism of the kinase domain of S-locus receptor kinase (SRK) which participates in the self-incompatibility of Brassicaceae were analyzed by polymerase chain reaction (PCR)-restriction fragment length polymorphism and nucleotide sequencing. In the PCR experiment, a SRK-specific primer pair (KS 5 and KA 7) of the class Ⅰ amplified a single DNA fragment of about 900 bp from 61 lines out of 134 inbred lines. Four groups were found in a double digestion with HintⅠ and EcoRⅡ using the class Ⅰ SRK-specific primer pairs.
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