In Vitro Differentiated Functional Cardiomyocytes from Parthenogenetic Mouse Embryonic Stem Cells
2006
Shin, H.A. (Maria Infertility Hospital Medical Institute/Maria Biotech, Seoul, Republic of Korea) | Kim, E.Y. (Maria Infertility Hospital Medical Institute/Maria Biotech, Seoul, Republic of Korea) | Lee, K.S. (Maria Infertility Hospital Medical Institute/Maria Biotech, Seoul, Republic of Korea) | Cho, H.Y. (Maria Infertility Hospital Medical Institute/Maria Biotech, Seoul, Republic of Korea) | Lee, W.D. (Maria Infertility Hospital, Seoul, Republic of Korea) | Park, S.P. (Maria Infertility Hospital Medical Institute/Maria Biotech, Seoul, Republic of Korea), E-mail: [email protected] | Lim, J.H. (Maria Infertility Hospital, Seoul, Republic of Korea)
This study was conducted to examine whether the parthenogenetic mouse embryonic stem (P-mES) cells can differentiate into functional cardiomyocytes in vitro similar to (mES) cells. P-mES04 and IVF-derived mES03 cells were cultured by suspension culture for 4 days. The formed embryoid bodies (EBs) were treated with 0.75% dimethyl sulfoxide (DMSO) for further 4 days(4-/4+), and then plated onto gelatin coated culture dish. The appearance of contracting cardiomyocytes from the P-mES03 and mES03 cells was examined for 30 days.
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