Ectopic Expression of an Arabidopsis H+/Ca²+ Antiporter Gene in Bottle Gourd (Lagenaria siceraria)
2005
Han, J.S. (National Horticultural Research Institute, RDA, Suwon, Republic of Korea) | Kim, C.K. (Sangju National University, Sangju, Republic of Korea) | Oh, D.G. (National Horticultural Research Institute, RDA, Suwon, Republic of Korea) | Woo, J.G. (National Horticultural Research Institute, RDA, Suwon, Republic of Korea) | Kim, J.H. (National Horticultural Research Institute, RDA, Suwon, Republic of Korea)
An Arabidopsis thaliana H+/cation antiporter gene (CAX2-B) was introduced into bottle gourd (Lagenaria siceraria) by Agrobacterium-meduated transformation. Transgenic plants were obtained at frequencies of 0.8% under the selection pressure of 100 mgㆍL-¹ kanamycin monosulfate, in which integration and transcription of the transgenes were confirmed by polymerase chain reaction (PCR), Southern blot, and reverse transcriptional PCR analyses. The roots of T₁ plants expressing CAX2-B grown hydroponically contained 8.7% more Ca than control plants. The Mn contents were not significantly different. In addition to CAX2-B expression, nptⅡ evenly expressed kanamycin resistance in the T₁ plants.
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