Sequence analysis and expression of groE gene encoding heat shock proteins of Brucella abortus isolates
2005
Kim, T.Y. (ChoongAng Vaccine Laboratory, Daejeon, Republic of Korea) | Kim, J.Y. (Chungnam National University, Daejeon, Republic of Korea) | Chang, K.S. (University of Kentucky, Lexington, KY, USA) | Han, H.R. (Seoul National University, Seoul, Republic of Korea) | Kim, M.C. (Chungnam National University, Daejeon, Republic of Korea) | Park, C.S. (Chungnam National University, Daejeon, Republic of Korea) | Jun, M.H. (Chungnam National University, Daejeon, Republic of Korea), E-mail: mhjun@cnu.ac.kr
GroE that is a heat shock protein composed of GroEL and GroES is known as an immunodominant target of both the humoral and cellular immune responses in bovine brucellosis. This study was carried out to characterize groE gene encoding heat shock proteins of B. abortus isolated in Korea and to evaluate the immunogenicity of the GroE protein expressed in E. coli system. In PCR the specific signals with the size of 2,077 bp were detected in five strains isolated from the mammary lymphnodes of the dairy cattle that were serologically positive and the reference strains.
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