Relationship of Cotyledon Number with Procambium Differentiation in Somatic Embryogenesis of Codonopsis lanceolata L.
2005
Choi, P.S. (Nambu University, Kwangju, Republic of Korea), E-mail: [email protected] | Soh, W.Y. (Daejeon Health College, Daejeon, Republic of Korea) | Cho, D.Y. (Woosuk University, Jeonju, Republic of Korea) | Liu, J.R. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea)
Embryogenic callus was obtained from cotyledonary explants of Codonopsis lanceloata on Murashige and Skoog's medium supplemented with 1 mg/L 2,4-D. Suspension cultures of the embryogenic calli were grown on a shaker at 100 strokes/min, and then the calli were subcultured for 2 weeks in 2,4-D-free medium to produce somatic embryo. In somatic embryos at the globular stage, cotyledon initials began to differentiate themselves in the near distal end of the procambial strand.
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