Molecular Sexing Using SRY and ZF Genes in Pigs
2005
Cho, I.C. (National Institute of Subtropical Agriculture, RDA, Cheju, Republic of Korea) | Oh, M.Y. (Cheju National University, Jeju, Republic of Korea) | Kang, S.Y. (National Institute of Subtropical Agriculture, RDA, Cheju, Republic of Korea) | Lee, S.S. (National Institute of Subtropical Agriculture, RDA, Cheju, Republic of Korea) | Choi, Y.L. (National Institute of Subtropical Agriculture, RDA, Cheju, Republic of Korea) | Ko, M.S. (National Institute of Subtropical Agriculture, RDA, Cheju, Republic of Korea) | Han, S.H. (National Institute of Subtropical Agriculture, RDA, Cheju, Republic of Korea), E-mail: [email protected]
A method for sex determination of pigs was examined using polymerase chain reaction(PCR). Sex determining region Y(SRY) gene encoded on Y chromosome plays a key role for primary male development. Zinc finger X-Y(ZFX-ZFY) gene, one of the X-Y homology gene group was found on the X and Y chromosomes, respectively. We tested for molecular sexing by amplification patterns of SRY and ZF genes. Genomic DNAs from various resources including porcine hairs and semen collected from domestic pig breeds and native pigs was used for PCR assay of each gene. The amplified products for porcine SRY gene were yielded only in males but not in females.
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