Chilling tolerance of cucumber during germination and expression of lysine decarboxylase gene
2005
Lu Minghui,Li Xiaoming,Chen Jinfeng
chinois. 经15℃低温诱导，采用cDNA-AFLP技术从耐冷性强的黄瓜品种长春密刺中分离到一条特异片段，该片段在耐冷性弱的北京截头中不能被诱导，命名为cctr132。将cctr132回收测序并翻译为氨基酸序列，用blastx和blsatp程序在NCBI GenBank数据库中进行同源性检索和相似性比对，结果发现cctr132与水稻推测的类赖氨酸脱羧酶蛋白同源性为88.37%，相似性为100%，并且在CCTR132中检测到了赖氨酸脱羧酶家族推测的保守结构域PGGXGTXXE，说明黄瓜发芽期耐冷性与赖氨酸脱羧酶基因表达有关。
Afficher plus [+] Moins [-]anglais. With cDNA-AFLP technique, a specific fragment was isolated from a chilling tolerant cucumber cultivar ‘Changchunmici’, under induction at low temperature (15℃). This fragment could not be induced in the chilling sensitive cucumber cultivar ‘Beijing jietou’ and named as cctr132. After recollection, sequencing and translation, the results of blastx and blastp in GenBank of NCBI indicated that cctr132 had 88.37% identities and 100% positives with Oryza sativa putative lysine decarboxylase-like protein, respectively, and PGGXGTXXE, the putative conserved domain of lysine decarboxylase family, was detected from CCTR132, which suggested that the cucumber chilling tolerance during germination is related to the expression of lysine decarboxylase gene.
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