Comparative study on partial sequence of follicle stimulating hormone beta subunit gene from four goat breeds
2006
Li Li | Zhang Hongping | Wu Dengjun
chinois. 利用牛、绵羊等动物卿亚基基因序列设计引物,采用PCR法扩增并测定了马头山羊(2只)、昭通山羊(2只)、榕江小香羊(1只)以及雷州山羊(3只)4个品种脚亚基基因部分序列(GenBank登录号:AY853276)。在测定出的唧亚基基因732bp序列中,包括第一外显子全序列(1~61bp),第一内含子全序列(62~703bp)以及第二外显子部分序列(704-732bp),并且序列中A+T含量(66.12%)高于G+C(33.88%)。测定出的第二外显子部分编码序列(21bp)共编码7个氨基酸。序列比对发现,除了一只昭通山羊的第78位为碱基A以外,其余个体的此位点碱基缺失,此位点与产羔率之间的关系需做进一步的研究。[
Afficher plus [+] Moins [-]anglais. According to the FSHβ gene sequences of bovine and sheep, the primers are designed and the partial sequences of FSHβ gene from 2 Matou goats, 2 Zhaotong goats, 1 Rongjiang-xiao-xiang goat and 3 Leizhou goats are studied (Accession No. AY853276). It includes the complete first exon ( 1 -61 bp), first intron (62--703 bp) and partial sequence of exon 2 (704--732 bp) in the 732 base pairs of FSHβ gene sequence, and the content of A+ T (66.12%) is higher than G+ C (33.88%). The coding sequence of exon 2 codes 7 amino acids. Only one sequence from a Zhaotong goat has A at 78th loci, others lack this nucleotide, the relationship between the mutation loci and the birth rate is to be studied deeply in the future.
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